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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of <t>p-IKK</t> α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.
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Image Search Results


SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting macrophagic SHP2 for ameliorating osteoarthritis via TLR signaling

doi: 10.1016/j.apsb.2022.02.010

Figure Lengend Snippet: SHP099 inhibited Toll-like signaling (TLR) pathway, including its downstream pathways, including nuclear factor kappaB (NF- κ B) pathway, and PI3K–AKT pathway. (A) Schematic diagram for RNA-sequence analysis. (B) Venn diagram. (C) Encyclopedia of Genes and Genomes (KEGG) analysis of the biological function of differentially expressed genes (DEGs). (D) DEGs in the TLR signaling pathway. (E) DEGs in the NF- κ B signaling pathway. (F) DEGs in the PI3K–AKT signaling pathway. (G) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in RAW264.7 cells. (H) Western blot analysis of p-IKK α / β , IKK α / β , p-P65, P65, p-AKT, AKT, GAPDH after LPS stimulation with or without SHP099 treatment in BMDMs.

Article Snippet: The following antibodies were used: mouse anti-F4/80 (Santa Cruz), rabbit anti-iNOS (Cell Signaling Technology), mouse anti-CD80 (Invitrogen Antibodies), rabbit anti-CD206 (Abcam), rabbit anti-SHP2 (Cell Signaling Technology), rabbit anti-COL2 (Abcam), rabbit anti-COL10 (Abcam), rabbit anti-MMP3 (Proteintech), rabbit anti-COX2 (Cell Signaling Technology), rabbit anti-GAPDH (Cell Signaling Technology), rabbit anti-p-P65 (Cell Signaling Technology), rabbit anti-P65 (Cell Signaling Technology), rabbit anti- β -actin (Cell Signaling Technology), rabbit anti-histone H3 (Cell Signaling Technology), rabbit anti-p-AKT (Cell Signaling Technology), rabbit anti-AKT (Cell Signaling Technology), and rabbit anti-p-IKK α / β (Cell Signaling Technology).

Techniques: Sequencing, Western Blot